FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (1): 16-19.doi: 10.7506/spkx1002-6630-201201002

• Basic Research • Previous Articles     Next Articles

Rapid Analysis of Antioxidant Constituents from Taxillus delavayi, a Yi Ethnomedicinal Material

PIAO Xiang-lan1, DENG Zhang-qin1,CHEN Hu-biao2,WANG Jin1   

  1. 1. Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China;
    2. School of Chinese Medicine, Hong Kong Baptist University, Hongkong, China
  • Received:2011-02-15 Revised:2011-12-22 Online:2012-01-15 Published:2012-01-12
  • Contact: PIAO Xiang-Lan E-mail:xlpiao@163.com

Abstract:

Objectives: To rapidly separate and identify antioxidant constituents from the whole plant of Taxillus delavayi, a Yi ethnomedicinal material, and assess their antioxidant activity. Methods: The antioxidant activity of the methanol extract of the medicinal material and its different solvent fractions obtained by repeated extractions sequentially with dichloromethane, n-butanol and water was assessed by DPPH free radical scavenging assay. The dichloromethane-soluble fraction was further separated by silica gel column chromatography, and a high antioxidant component named as TDD8 was obtained and analyzed by bioassay-linked HPLC and 1H-NMR. Results: An individual compound named as TDD8-2 was separated from TDD8 by semi-preparative HPLC and identified by 1H-NMR as pinoresinol. Conclusion: The bioassay-linked HPLC method allows the rapid separation and identification of antioxidant components from medicinal materials.

Key words: Taxillus delavayi, DPPH radical scavenging activity, bioassay-linked HPLC method, pinoresinol

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