Isolation, Purification and Structural Analysis of Fucoidan from Kelp

doi:10.7506/spkx1002-6630-201201013

FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (1): 68-71.doi: 10.7506/spkx1002-6630-201201013

• Basic Research • Previous Articles Next Articles

Isolation, Purification and Structural Analysis of Fucoidan from Kelp

ZHANG Wen-qing，ZUO Ping-ping，XU Chen，YANG Lei，ZHANG Yu-long，XIA Wei*

Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering,
East China University of Science and Technology, Shanghai 200237, Chinaa

Received:2011-01-17 Revised:2011-12-27 Online:2012-01-15 Published:2012-01-12
Contact: XIA Wei E-mail:zuopp123@163.com

Abstract

Abstract:

A homogeneous polysaccharide named as TC-1 was obtained from kelp by DEAE-cellulose and gel filtration chromatography. Its chemical structure was characterized by means of monosaccharide composition analysis, methylation analysis and IR spectroscopic analysis. TC-1 was a complex sulfated polysaccharide with a weight-average molecular weight of 3.7 × 105. TC-1 mainly consisted of fucose and small amounts of xylose, mannose, glucose and galactose were also found. The chain of TC-1 included 1,4-linked or 1,3-linked fucose, 1,3-linked xylose, 1,3-linked or 1,6-linked mannose, 1,3,4-linked or 1,2,4,6-linked glucose, and 1,6- linked,1,3,6- linked, or 1,3,4,6- linked galactose.

Key words: kelp, fucoidan, isolation and purification, structural analysis

CLC Number:

O636.1

ZHANG Wen-qing，ZUO Ping-ping，XU Chen，YANG Lei，ZHANG Yu-long，XIA Wei. Isolation, Purification and Structural Analysis of Fucoidan from Kelp[J]. FOOD SCIENCE, 2012, 33(1): 68-71.

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Isolation, Purification and Structural Analysis of Fucoidan from Kelp

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