Abstract: Scallop gonad polysaccharide extract (SGP) was obtained by hydrolysis with a series of commercial enzymes. Its antioxidant activity was evaluated by DPPH system, Fenton system and reduction reaction. MTT assay was used to evaluate its effect on tBOOH-induced oxidative damage and lymphocyte proliferation. The effect on complement activity was evaluated by spectrophotometry. The results indicated that SGP had reducing power and scavenging effect on DPPH and hydroxyl free radicals. SGP at the 0.2μg/mL could also protect RAW264.7 cells from oxidative damage induced by tBOOH. Moreover, SGP could also promote lymphocyte proliferation and activate complement activity.

Key words: scallop gonad, polysaccharide extracts, antioxidant, lymphocyte proliferation, complement