Abstract: A reversed-phase high performance liquid chromatographic method with solid-phase extraction was proposed for the residue dynamic analysis of imazalil in rambutan. The analyte was separated on a Shim-pack VP-ODS (150 mm × 4.6 mm, 5μm) using a mobile phased composed of a mixture at a volume ratio of 75:25 of methanol and acidic Na2H2PO4 solution approximately at pH 5 (adjusted with 85% phosphoric acid) at a flow rate of 0.85 mL/min, and detected at 229 nm. The injection amount was 20 μL. An excellent linearity of imazalil in the range of 15.0－960.0 μg/L was achieved (r ＝0.9994). The detection limit was 5.0 μg/L. The average spike recovery rates were in the range of 86.4%－104.5% with relative standard deviation of 0.84%－2.87%. The method was fast, simple, and applicable for the determination of imazalil residue in rambutan.

Key words: imazalil, solid-phase extraction, RP-HPLC, rambutan