Physico-chemical Properties and Antioxidant Activity in vitro of Intracellular Polysaccharides from Phellinus igniarius

doi:10.7506/spkx1002-6630-201209008

Abstract

Abstract: In this study, physicochemical properties and antioxidant activity of intracellular polysaccharides (IPs) isolated from liquid mycelial culture of Phellinus igniarius were analyzed. Crude IPs extract was separated into 8 fractions by DEAE-52 cellulose anion-exchange column chromatography, most of which were high molecular weight neutral polysaccharides. Gel permeation chromatographic (GPC) analysis showed that the average molecular weight (MW) of IPs ranged from 5.7 × 103 D to 6.1 × 106 D. The monosaccharide composition consisted of glucose, mannose, and galactose at a molar ratio of 15:4:1, the polysaccharide content in crude IPs extract was 41% and its intrinsic viscosity was 12 mL/g. IPs from Phellinus igniarius mycelia could effectively scavenge hydroxyl and superoxide anion free radicals and chelate Fe2+. Furthermore, more obvious scavenging activity was observed against superoxide anion free radicals than hydroxyl free radicals.

Key words: Phellinus igniarius, intracellular polysaccharides(IPs), physico-chemical properties, antioxidant activity in vitro

CLC Number:

TQ929.2

YANJing-kun，MAHai-le，ZHUZi-ping，ZHENGHui-hua，YANGXiao-ming，LIUWei-min. Physico-chemical Properties and Antioxidant Activity in vitro of Intracellular Polysaccharides from Phellinus igniarius[J]. FOOD SCIENCE, 2012, 33(9): 36-40.

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Physico-chemical Properties and Antioxidant Activity in vitro of Intracellular Polysaccharides from Phellinus igniarius

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