FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (9): 114-117.doi: 10.7506/spkx1002-6630-201209024

• Basic Research • Previous Articles     Next Articles

Effect of PEF Treatment on Secondary Structure of Ovalbumin under Various pH Conditions

YANG Mei-fang,ZENG Xin-an*,LIU Yan-yan   

  1. (College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China)
  • Online:2012-05-15 Published:2012-05-07

Abstract: The effect of high intensity pulsed electric field (HIPEF) treatment on the secondary structure of ovalbumin under various pH conditions was explored using circular dichroism (CD) spectroscopy. The results indicated that 4 circles of PEF treatment (192μs) at the field intensity of 26.7 kV/cm could induced the unfolding of α-helix structure by 25.43%, 23.99% and 42.20%, as well as unfolding of β-sheet structure by 26.20%, 15.99% and 38.53% under the conditions of pH 3, 7 and 12, respectively.

Key words: high intensity pulsed electric field (HIPEF), pH, ovalbumin, secondary structure, circular dichroism spectroscopy

CLC Number: