Coenzyme Q10 Attenuates Platelet Thromboxane A2 Generation through Regulating the Protein Kinase A/Cytosolic Phospholipase A2 Signaling Pathway

doi:10.7506/spkx1002-6630-20200608-108

FOOD SCIENCE ›› 2021, Vol. 42 ›› Issue (9): 130-136.doi: 10.7506/spkx1002-6630-20200608-108

• Nutrition & Hygiene • Previous Articles Next Articles

Coenzyme Q10 Attenuates Platelet Thromboxane A2 Generation through Regulating the Protein Kinase A/Cytosolic Phospholipase A2 Signaling Pathway

YA Fuli, ZHANG Chunmei, CHEN Binlin, GU Shiyan, JIA Xiao’e

(1. Institute of Preventive Medicine, School of Public Health, Dali University, Dali 671000, China; 2. Hekou Customs of the People’s Republic of China, Hekou 661300, China;3. Department of Nutrition, Maternity and Child Health Care of Guangxi Zhuang Autonomous Region, Nanning 530000, China)

Online:2021-05-15 Published:2021-06-02

Abstract

Abstract: Objective: Thromboxane A2 (TxA2) derived from platelets can facilitate atherosclerosis and thrombosis. Previous studies have demonstrated that coenzyme Q10 (CoQ10) attenuates platelet activation, aggregation and thrombus formation. Our present study aimed to investigate the effect of CoQ10 on TxA2 generation and to clarify the underlying mechanisms. Methods: Gel-filtered platelets prepared from heathy adults were incubated with different concentrations of CoQ10 (0, 1, 10 and 100 μmol/L) for 50 min. After agonist activation, the level of TxB2 secreted from platelets was determined by enzyme-linked immunosorbent assay. The phosphorylation of cytosolic phospholipase A2 (cPLA2) was measured by Western blot. Results: CoQ10 significantly inhibited platelet TxA2 generation induced by several agonists, including thrombin, collagen and convulxin. Western blot showed that CoQ10 significantly down-regulated thrombin- and collagen-induced cPLA2 phosphorylation. Moreover, pyrrophenone, a cPLA2 specific inhibitor, showed no any additive effects on TxA2 generation when combined with CoQ10. Furthermore, the inhibitory effect of CoQ10 on agonist-induced platelet cPLA2 phosphorylation and TxA2 generation was almost completely reversed by protein kinase A (PKA) inhibitor H89. Conclusion: CoQ10 can attenuate agonist-induced platelet TxA2 generation, and the mechanism is mainly through regulating the PKA/cPLA2 signaling pathway.

Key words: coenzyme Q10; platelet; thromboxane A2; cytosolic phospholipase A2; cardiovascular diseases

CLC Number:

R151.4

YA Fuli, ZHANG Chunmei, CHEN Binlin, GU Shiyan, JIA Xiao’e. Coenzyme Q10 Attenuates Platelet Thromboxane A2 Generation through Regulating the Protein Kinase A/Cytosolic Phospholipase A2 Signaling Pathway[J]. FOOD SCIENCE, 2021, 42(9): 130-136.

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Coenzyme Q10 Attenuates Platelet Thromboxane A2 Generation through Regulating the Protein Kinase A/Cytosolic Phospholipase A2 Signaling Pathway

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