FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (9): 144-148.doi: 10.7506/spkx1002-6630-201209030

• Bioengineering • Previous Articles     Next Articles

Screening and Protoplast Mutagenesis of Aspergillus niger for Enhanced Endoinulinase Activity

LI Wen-li,CAO Ze-hong*,DONG Yu-wei,SUN Hui-gang,LIU Xi-juan,TANG Pan   

  1. (College of Food and Bioengineering, Xuzhou Institute of Technology, Xuzhou 221008, China)
  • Online:2012-05-15 Published:2012-05-07

Abstract: Soil was collected from the periphery of rotten burdock roots in the burdock planting base of Qinzhuang village, Hekou town, Pei county, Xuzhou municipality. Three batches of Aspergillus niger strains (including Aspergillus niger 3.316 strains) were isolated and 17 strains with an endoinulinase (I)-to-exoinulinase (S) activity ratio of higher than 10 were screened from them by secondary screening. Strain C122803 exhibited the highest I/S ratio (2.78 U/mL) among 17 screened strains. Strain YY18 with an endoinulinase activity of 3.97 U/mL was obtained from strain C122803 through protoplast preparation and LiCl mutagenesis. The activity obtained was 42.80% higher than that of the parent strain.

Key words: Aspergillus niger, inulase, protoplast, mutagenesis

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