FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (12): 12-17.doi: 10.7506/spkx1002-6630-201212003

• Processing Technology • Previous Articles     Next Articles

Optimization of Lipase-Catalyzed Synthesis of Ethyl Hexanoate in Solvent Free System by Response Surface Methodology

ZHAO Lei,CHENG Yue,WANG Cheng-tao*   

  1. (Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing Key Laboratory of Flavor Chemistry, Beijing Technology and Business University, Beijing 100048, China)
  • Online:2012-06-25 Published:2012-07-27

Abstract: Objective: To optimize lipase-catalyzed synthesis of ethyl hexanoate in a solvent-free system. Methods: One-factor-at-a-time experiments were cairred out to identify lipase amount, molar ratio of hexanoic acid to ethanol and reaction time as main variables that influence ethyl hexanoate yield for response surface optimization based on a three-vairable, five-level central composite design. Results: The optimum conditions for lipase-catalyzed synthesis of ethyl hexanoate were determined as 0.5% of enzyme amount, 1:1.3 of molar ratio of hexanoic acid to ethanol, 12.2 h of reaction time and 30 ℃ of reaction temperature, and 150 r/min of rotary speed. Under these conditions, the experimental value of ethyl hexanoate yield was 81.6%, which was close to the predicted value (87.7%). Conclusion: It is feasible to optimize lipase-catalyzed synthesis of ethyl hexanoate in a solvent-free system by response surface methodology.

Key words: ethyl hexanoate, response surface analysis, central composite design, solvent-free system, lipase-catalyzed synthesis

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