Structural Changes of Common Carp Myofibrillar (MPI) Influenced by Hydroxyl Radical System

doi:10.7506/spkx1002-6630-201213013

Abstract

Abstract: The objective of this study was to investigate the structural changes of common carp myofibrillar (MPI) after exposure to hydroxyl radical-generating systems (HRGS) containing 0.1 mmol/L ferric chloride, 0.1 mmol/L ascorbic acid and 0－20 mmol/L hydrogen peroxide for 1, 3 h and 5 h, respectively. Hydroxyl radical oxidation caused structural changes in fish MPI. As a result, the carbonyl groups content and hydrophobicity increased, while active sulfhydryl groups content and protein solubility decreased. The SDS-PAGE results showed that protein oxidation could cause protein aggregation by cross-linking between proteins. These oxidation-induced structural changes reveal high susceptibility of common carp MPI to oxidative stress.

Key words: carp, myofibrillar MPI, protein oxidation, structure

CLC Number:

TS254.4

LIYan-qing，KONGBao-hua，YANGHe-hong，XIAXiu-fang. Structural Changes of Common Carp Myofibrillar (MPI) Influenced by Hydroxyl Radical System[J]. FOOD SCIENCE, 2012, 33(13): 70-74.

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Structural Changes of Common Carp Myofibrillar (MPI) Influenced by Hydroxyl Radical System

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