Purification and Homology Analysis of Enterococcin SAU-2

doi:10.7506/spkx1002-6630-201213038

Abstract

Abstract: Enterococcin SAU-2 was partially purified by 90% ammonium sulfate precipitation followed by SP Sepharose Fast Flow chromatography. After the purification of enterococcin, a 10.79-fold enhancement in specific activity and a recovery rate of 17.27% were achieved. The estimated molecular weight of enterococcin SAU-2 was approximately 4350 D based on Tricine-SDS-PAGE. The structural gene and amino acid sequences of the enterococcin SAU-2 had low homology with those of most enterococcins. The enterococcin SAU-2 was identified as a new member of the bacteriocin family.

Key words: enterococcin, purification, homology analysis

CLC Number:

S852.611
TS201.3

HUXin-jie，ZHOUJia，LIUShu-liang. Purification and Homology Analysis of Enterococcin SAU-2[J]. FOOD SCIENCE, 2012, 33(13): 180-182.

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Purification and Homology Analysis of Enterococcin SAU-2

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