FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (24): 66-71.doi: 10.7506/spkx1002-6630-201224014

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Optimization of Simultaneous Extraction of Proteins and Polysaccharides from Undaria pinnatifi da by Response Surface Methodology

WANG Ming-yan,ZHOU Qin,YANG Jun,CAI Xiao-qing,TAN Qi,ZHANG Qiu-rong   

  1. Jiangsu Marine Resoures Development Research Institute, Huaihai Institute of Technology, Lianyungang 222005, China
  • Received:2011-09-24 Revised:2012-11-21 Online:2012-12-25 Published:2012-12-12

Abstract:

Response surface methodology was used to optimize the simultaneous alkaline extraction of proteins and polysaccharides from Undaria pinnatifi da based on extraction effi ciency. All four operating parameters including alkaline concentration, extraction time, extraction temperature and solvent-to-solid ratio on had a signifi cant effect on the extraction yields of proteins and polysaccharides. The optimum process conditions for simultaneously increasing the yields of proteins and polysaccharides were found to be extraction at 55 ℃ for 5 h with an alkaline concentration of 2.2% and a solvent-tosolid ratio of 23:1 (mL/g). The actual yields of proteins and polysaccharides under the optimized conditions were 63.21% and 29.11%, respectively. Good agreement with the predicted values was found. Therefore, response surface methodology is a feasible way to optimize the simultaneous alkaline extraction of proteins and polysaccharides from Undaria pinnatifi da.

Key words: Undaria pinnatifida, protein, polysaccharides, extraction, response surface methodology, overlay contour plot

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