FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (24): 219-221.doi: 10.7506/spkx1002-6630-201224045

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Development of a Real-Time Fluorescent RT-PCR Assay for Detection of Infectious Bronchitis Virus

CHEN Jun，ZHOU Guang-ming*，YANG Yuan-gao，ZHANG Li-xian   

  1. Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education,College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China
  • Received:2011-12-23 Revised:2012-11-13 Online:2012-12-25 Published:2012-12-12

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Abstract: A real-time PCR method was developed for the specific, sensitive and rapid detection of infectious bronchitis virus (IBV). A pair of specific primers and a specific probe were designed according to the IBV gene conserved sequence. The specificity, sensitivity and repeatability of this method were analyzed. This method was shown to have good specificity and repeatability and the minimum detection of IBV DNA copy number was 5. This RT-PCR assay showed high IBV specificity, sensitivity and time-saving and thus could provide a helpful tool for the prognosis of IBV.

Key words: infectious bronchitis virus (IBV), fluorescent RT-PCR

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