Process Optimization by Response Surface Methodology for the Extraction of Water Soluble Flavonoids from Coreopsis tinctoria Flowers

doi:10.7506/spkx1002-6630-201306028

Abstract

Abstract: Response surface methodology (RSM) was applied to optimize the extractions of water soluble flavonoids from Coreopsis tinctoria flowers using water as the extraction solvent. The effects of extraction temperature, material/liquid ratio, particle size and extraction time on the extraction efficiency of water soluble flavonoids were investigated. The optimal extraction conditions were extraction temperature of 90 ℃, material/liquid ratio of 1:90, extraction time of 60 min and raw material particle size of 40 mesh. The actual extraction rate of flavonoids from Coreopsis tinctoria under the optimal extraction conditions was 24.85%, which was close to the predicted value.

Key words: Coreopsis tinctoria, lavonoids, extraction, response surface methodology (RSM)

CLC Number:

TS201.1

References

[1] 新疆植物志编辑委员会.新疆植物志(第5卷)[M].乌鲁木齐:新疆科技卫生出版社.1999:93.
[2] 刘伟新, 邓继华, 徐鸿.一种金鸡菊花的生药学研究[J].中国民族医药杂志,2009,1:24-25.
[3] Jeffry B H, Christine A V. Advances in flavonoid research since 1992[J]. Phytochemistry,2000,55:481-504.
[4] Bent H H. The biochemistry and medical significance of the flavonoids [J].Pharmacology &Therapeutics,2002,96:67-202.
[5] Chang W T, Shao Z H, Yin J J, et al.Comparative effects of flavonoids on oxidant scavenging and ischemia-reperfusion injury in cardiomyocytes [J]. Eur J Pharmacol,2007,566(1-3):58-66
[6] YI Zhibiao, YU Yan, LIANG Yizeng, et al. In vitro antioxidant and antimicrobial activities of the extract of Pericarpium Citri Reticulatae of a new Citrus cultivar and its main flavonoids[J]. LWT-Food Science and technology, 2008, 41(4): 597-603.
[7] 张氽,阚建全,陈宗道.生物类黄酮抗癌作用研究进展[J].中国食品添加剂,2003,13:17-20.
[8] Lu J , Papp L V, Fang J G, et al. Inhibition of mammalian thioredoxin reduce tase by some flavonoids: implications for myricetin and quercetin anticancer activity [ J ]. Cancer Res ,2006, 66 (8) : 4410-4418.
[9] 朱笃, 陈飞彪, 夏剑辉.金鸡菊总黄酮提取及含量测定[J]. 食品科学, 2005, 26(9): 314-316.
[10] 周瑞雪, 阎志惠, 刘恩荔, 等.苦荞黄酮类化合物的提取工艺[J].中药材, 2006,8(6):45-49.
[11] 马菁,幸让新,王华强.恰玛古中类黄酮的提取工艺研究[J].食品科技,2010,35(5)：208-213.
[12] 张玉,曾凡坤,吴剑.响应面法优化柑橘皮渣中类黄酮的超声波提取工艺[J]. 食品科学,2010, 28,31( 08)：28-32.
[13] 范莹莹, 黄小凤.银杏叶中黄酮类化合物的提取工艺研究[J].云南化工, 2006,8(2):21-23.
[14] 朱宏莉, 韦海洪, 宋纪蓉, 等.竹叶类黄酮的提取和纯化工艺的研究[J].食品科学, 2005, 26(8):158-161.
[15] 吴华勇, 黄赣辉, 顾振宇.响应曲面法优化竹叶总黄酮的提取工艺研究[J].食品科学, 2008,29(11):196-200.
[16] 刘峙嵘, 俞自由, 方裕勋, 等.微波萃取银杏叶黄酮类化合物[J].东华理工学院学报, 2005,28(2):151-154.