FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (7): 266-269.doi: 10.7506/spkx1002-6630-201307056

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Protective Effect of Selenium-Enriched Polysaccharides from Cordyceps militaris against Rotenone-Induced Oxidative Damage in Drosophila melanogaster

SUN Jun-de,HOU Jing,YANG Yi   

  1. College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, China
  • Received:2012-03-05 Revised:2013-03-05 Online:2013-04-15 Published:2013-03-20
  • Contact: SUN Jun-de E-mail:sunjunde108@163.com

Abstract: Objective: To explore the protective effect of Cordyceps militaris polysaccharide (CPS) and selenium-enriched polysaccharide (SE-CPS) on rotenone-induced oxidative stress and neurotoxicity in Drosophila melanogaster. Methods: Rotenoneinduced fruit fly damage models were divided into basic medium with or without CPS and Se-CPS to evaluate oxidative stress and neurotoxity. Seven antioxidant indicators including malondialdehyde (MDA), protein carboxyl (PC), glutathione content (GSH and GSSG), superoxide dismutase (SOD) and glutathione-S-transferase (GST) were measured. Meanwhile, acetylcholinesterase (Ache,) and butyrylcholinesterase (Bche) activities were also analyzed to evaluate the neuroprotective role of both polysaccharides. Results: Both CPS and Se-CPS could reduce oxidative stress indicators and neurotoxicity. Se-CPS had better protective effect at equal concentrations; 1% Se-CPS could restore the damage to 85% level of control group. Moreover, some oxidative stress indicators and enzyme activity were also restored to the control level.

Key words: Cordyceps polysaccharide, selenium, rotenone, antioxidant, neuroprotective

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