Determination of Isoflavones in Soybean Products and Supplements by UPLC

doi:10.7506/spkx1002-6630-201308034

Abstract

Abstract: Objective: To develop an ultra-high performance liquid chromatography (UPLC) method for the determination of four major isoflavones such as daidzin, genistin, daidzein and genistein in soybean products and supplements. Methods: The analytes were separated on an Acquity UPLC BEH C18 column (50 mm × 2.1 mm, 1.7 μm) with a mobile phase consisting of 0.2% formic acid and acetonitrile in gradient mode, and UV detection at 254 nm. The flow rate was 0.4 mL/min. The column temperature was 30 ℃. Results: A good linear relationship within the range of 0.625 to 40.0 mg/L for daidzin (r = 1.000), 0.625 to 40.0 mg/L for genistin (r = 0.9999), 0.04 to 2.56 mg/L for daidzein (r = 0.9997) and 0.04 to 2.56 mg/L for genistein (r = 0.9996) was observed. The average recovery rates of daidzin, genistin, daidzein and genistein were 98.5%–99.8%. Conclusion: The developed method is fast, simple, sensitive, and accurate for the determination of four isoflavones in soybean products and supplements.

Key words: ultra-high performance liquid chromatography (UPLC), soybean products, isoflavone, quantitative determination

CLC Number:

TS214.2

YUAN Bo，ZHEN Hui-juan，JIANG Xuan，SUN Shuai-ting，XU Li，XU Hai-yan. Determination of Isoflavones in Soybean Products and Supplements by UPLC[J]. FOOD SCIENCE, 2013, 34(8): 164-167.

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