Abstract:

Purpose: To optimize the hydrolysis (into genistein) of isoflavones extracted from Fructus Sophorae with “Amano”
β-glucosidase. Methods: The optimization of four hydrolysis conditions including enzyme concentration, time, pH and
temperature for improved hydrolysis efficiency was performed using response surface methodology (RSM). Results: The
optimal hydrolysis conditions were determined as follows: enzyme concentration, 0.5 mg/mL; hydrolysis time, 3.68 h; pH
value, 4.95, and hydrolysis temperature, 59.2 ℃. Under these conditions, the hydrolysis efficiency of total isoflavones was as
high as 95.76%. Our investigation is significant for the exploitation and application of medicinal pants.

Key words: Fructus Sophorae isoflavones, enzymatic hydrolysis, high performance liquid chromatography