Over-expression of Lactate Dehydrogenase in L.casei and Fermentation Property of the Recombinant Strain

doi:10.7506/spkx1002-6630-201309050

Abstract

Abstract:

Lactate dehydrogenase (LDH) is a key enzyme for lactic acid synthesis in L. casei. A LDH over- expressing
recombinant L.casei was constructed in this study to enhance the production of lactic acid. The lactate dehydrogenase gene ldh
of L. casei was first cloned into vector pMG36e. The recombinant vector was then transferred into L. casei. SDS-PAGE analysis
showed that LDH was successfully over expressed in the recombinant strains, which was about 37 kD. Enzyme activity analysis
showed that LDH activity of the recombinant strain increased by 3.1 times when compared to that of the wild type strain.
4 mmol/L activator FBP and 8 mg/L nicotinic acid were then added to fermentation medium to further improve lactic acid production
and analysis showed that accumulation of lactic acid was 128.2 g/L, and the lactic acid productivity was 3.04 g/(L•h), which were
increased by 35% and 53%, respectively, when compared to control. Addition of FBP and nicotinic acid significantly increased
the consumption rate of glucose and shortened fermentation time by 6 h.

Key words: LDH, recombinant L.casei, over-expression, fermentation characteristics

CLC Number:

ZHU Long-bao，GE Fei，LI Wan-zhen，TAO Yu-gui*. Over-expression of Lactate Dehydrogenase in L.casei and Fermentation Property of the Recombinant Strain[J]. FOOD SCIENCE, 2013, 34(9): 245-249.

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