Abstract:

This paper reviews the recent progress in the purification, characterization, molecular cloning and recombinant
expression of myofibril-bound serine proteinases (MBSP) from different species of fish. As a weakly alkaline proteinase,
MBSP is extensively found in fish skeletal muscles. The degradation of myofibrillar proteins by MBSP is mainly resposible
for the modori phenomenon during fish surimi production. From this reviwe, we conclude that fish-derived MBSP hold great
promise as a novel tool in mass spectrometric identification of proteins due to their excellent stablity to heat and substrate
specificity to decompose the carboxyl chains of arginine or lysine residues.

Key words: myofibril-bound serine proteinase, purification, characterization, molecular cloning, recombination expression