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Analysis of Bacterial Phylogenetic Diversity of Pit Muds with Different Cellar Ages

WANG Ming-yue,ZHANG Wen-xue,WANG Hai-ying,LIU Chao-lan   

  1. 1. Fuyang Vocational and Technical College, Fuyang 236031, China;2. College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, China
  • Online:2013-06-15 Published:2013-06-03

Abstract:

Total DNAs were extracted from pit muds with different cellar ages, 20, 50 a and 150 a, respectively and
were used for constructing 16S rDNA clone libraries. The bacterial composition and diversity from the pit muds were
investigated by amplified rDNA restriction analysis (ARDRA), DNA sequencing and phylogenetic analysis. The results
showed that 398 clones from the 16S rDNA library fell into 37 operational taxonomic units (OTUs) and 6 phyla including
Firmicute (65.1%), Bacteroidetes (21.4%), Chloroflexi (4.8%), Actinobacteria (3.8%), unclassified bacteria (3.9%) and
Proteobacteria (1.0%). Most of the clones (73.9%) showed more affiliation with the uncultured bacteria. The predominant
bacteria from the pit mud with cellar age of 20 years were Syntrophomonas, Petrimonas and Proteiniphilum, while the
predominant bacteria from the pit muds with cellar age of 50 a and 150 a were Natronoanaerobium and unclassified
Clostrida. The bacterial diversity index of pit mud increased with increasing cellar age, especially when the cellular age
increased from 20 to 50 a. The community structure of the pit muds with cellar age of 50 a and 150 a had the highest
similarity. This research revealed the relationship between bacterial phylogenetic diversity of pit muds with different
cellar ages and the aging of pit mud.

Key words: pit mud, 16S rDNA sequence, clone library, phylogenetic, diversity