Abstract:

This paper describes the development of a model for near-infrared spectral quantitative analysis of anthocyanins in
pomegranate. Pomegranate was tested with an InfraXact NIR analyzer. The effects of different pretreatment and calibration methods
on modeling were studied by orthogonal array design. A calibration model was developed and evaluated by objective function method
and the optimized model was validated with respect to applicability. Under optimal conditions, the coefficient of determination was
0.958, RSD 6.599%, and RPD 4.970. These results showed that NIRS could provide a feasible method for quantitative analysis of
anthocyanins in pomegranate and the model established in this study had good stability and prediction ability.

Key words: near infrared spectroscopy, pomegranate, quantitative analysis model, anthocyanins