Determination of Hydroxyl Radical Scavenging Capacity of Polysaccharides from Roots of Toddalia asiatica by Flow Injection Chemiluminescence Method

doi:10.7506/spkx1002-6630-201313017

FOOD SCIENCE

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Determination of Hydroxyl Radical Scavenging Capacity of Polysaccharides from Roots of Toddalia asiatica by Flow Injection Chemiluminescence Method

TIAN Chun-lian1，KANG Lian-chang2，WANG Peng1，GONG Zhu-qiong3

1. Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, China；
2. College of Chemical Technology, Huaqiao University, Xiamen 361021, China；3. Zhangjiajie Institute of Product Quality
Supervision and Inspection, Zhangjiajie 427000, China

Online:2013-07-15 Published:2013-06-28
Contact: TIAN Chun-lian

Abstract

Abstract:

The hydroxyl radical scavenging activity of polysaccharides from Toddalia asiatica roots was investigated by flow
injection chemiluminescence method and the effects of polysaccharide concentration, time, light and heat treatment on this
antioxidant activity were studied. Vitamin C and tea polyphenol (TP) were used as positive controls. The results showed that
the hydroxyl radical scavenging activity of TP as a potent antioxidant was significantly higher than that of Toddalia asiatica
polysaccharides and VC. The hydroxyl radical scavenging activity of of Toddalia asiatica polysaccharides showed a positive
concentration dependence over the range of 0.1 to 500 μg/mL and the scavenging rate at 500 μg/mL was as high as 94%. The
scavenging activity of VC was reduced substantially when exposed to strong light while Toddalia asiatica polysaccharides had
good light stability. Our results indicate that Toddalia asiatica is a promising natural source of antioxidants.

Key words: Toddalia asiatica polysaccharides, flow injection chemiluminescence method, hydroxy radical, scavenging rate

CLC Number:

Q946.3

TIAN Chun-lian1，KANG Lian-chang2，WANG Peng1，GONG Zhu-qiong3. Determination of Hydroxyl Radical Scavenging Capacity of Polysaccharides from Roots of Toddalia asiatica by Flow Injection Chemiluminescence Method[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201313017.

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Determination of Hydroxyl Radical Scavenging Capacity of Polysaccharides from Roots of Toddalia asiatica by Flow Injection Chemiluminescence Method

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