Abstract:

The plasmid pET28a-BMP-2 based on the target gene of bone morphogenetic protein-2 (BMP-2) from E.coli
harboring plasmid BMP-2cDNA was transformed into E.coli BL 21(DE3). The successful expression of BMP-2 in E.coli BL
21(DE3) was identified by enzymatic digestion. Under optimized expression conditions, BMP-2 was purified and renaturated
to obtain enhanced bioactivity. This study provides a cost-effective way of producing BMP-2, which is expensive and
needed in large quantities in bone repair materials and investigation of its sustained release capacity.

Key words: bone morphogenetic protein -2, inducement expression, pet-28 a(+), purification, renaturation