Optimization by Orthogonal Array Design of Separation and Purification of Chlorogenic Acid from Eucommia ulmoidues Leaves by Macroporous Adsorption Resin

doi:10.7506/spkx1002-6630-201316005

Abstract

Abstract:

Different types of macroporous adsorption resin, including NKA-2, NKA-9, LS-600, HZ806, HZ906,
HPD100, HPD500 and S-8 were compared for their static adsorption and desorption capacities for chlorogenic acid in
60% ethanol extract of Eucommia ulmoidues leaves. NKA-2 was found to have a stronger ability to adsorb and desorb
chlorogenic acid and the optimal operating parameters for purifying chlorogenic acid from the ethanol extract using
NKA-2 were determined. The static adsorption quantity of chlorogenic acid on NKA-2 and desorption percentage were
86 mg/g and 93.23%, respectively. The optimal process conditions that provided maximum chlorogenic acid recovery
(83.14%; purity 28.91%) were determined as 4 mg/mL, 4 BV/h, 5 BV, 80%, 3 BV/h and 2 BV for sample concentration,
loading flow rate, loading volume, desorbent concentration, desorption flow rate, and desorbent volume, respectively,
and whereas those for maximum chlorogenic acid purity (39.38%; recovery 79.62% ) were 4 mg/mL, 4 BV/h, 5 BV,
80%, 3 BV/h and 1.5 BV, respectively.

Key words: Eucommia ulmoidues leaves, chlorogenic acid, macroporous resin, separation and purification

XIONG Shuo，CUI Li，LIU Zhong-hua，ZHANG Sheng，XIAO Wen-jun. Optimization by Orthogonal Array Design of Separation and Purification of Chlorogenic Acid from Eucommia ulmoidues Leaves by Macroporous Adsorption Resin[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201316005.

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Optimization by Orthogonal Array Design of Separation and Purification of Chlorogenic Acid from Eucommia ulmoidues Leaves by Macroporous Adsorption Resin

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