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Isolation and Characterization of Collagens from Skin of Grass Carp (Ctenopharyngodon idellus) and Snakehead (Channa argus)

WANG Zhong-wen1,WANG Hai-bo1,*,LIANG Yan-ping1,LI Yun-yan1,WANG Min1,WANG Hai-ying2   

  1. 1. College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China;
    2. Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
  • Online:2013-09-15 Published:2013-09-27
  • Contact: WANG Hai-bo

Abstract:

In this study, the molecular structure and physico-chemical properties of collagens from herbivorous fish (grass
carp) and carnivorous fish (snakehead) were compared. Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC)
extracted from grass carp and snakehead skins were characterized comparatively for their compositional, structural and
physiochemical properties and thermal stability and the results obtained were compared with those of pig skin collagen. Results
indicated that all five extracted collagens were classified as type Ⅰ collagen which comprised two different α chains (α1 and
α2). Collagens from different sources had considerably varying amino acid composition and protein patterns as well as their
primary structure. The denaturation temperatures of different collagens were: PSC from pig skin (41.58 ℃), ASC from grass
carp skin (35.62 ℃), PSC from grass carp skin (35.81 ℃), ASC from snakehead skin (33.85 ℃) and PSC from snakehead
skin (34.06 ℃). The enzymatic hydrolyzability of different collagens decreased in the order of snakehead skin PSC, grass carp
skin PSC, snakehead skin ASC, grass carp skin PSC and pig skin PSC. Collagens extracted from grass carp and snakehead had
lower denaturation temperatures and higher enzymatic hydrolyzability than those from pig skin. Grass carp skin collagens were
more resistive to collagenase and showed higher denaturation temperature compared with snakehead skin collagens. In addition,
ASC was more resistive to collagenase compared to PSC.

Key words: collagen, grass carp, snakehead, isolation, physico-chemical properties

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