Abstract:

An enzymatic hydrolysis method for peeling sesame was proposed and the optimum hydrolysis parameters
were explored. The optimum enzymes and mixing ratio were screened out of cellulase, xylanase and pectinase by mixture
experimental design based on peeling efficiency and the hydrolysis conditions were optimized by one-factor-at-a-time and
orthogonal array designs. The optimum enzymatic hydrolysis conditions that provided maximum peeling efficiency (98.3%)
were determined as 1:3.6, 0.4%, 30 min and 50 ℃ for activity ratio between cellulase and xylanase, total enzyme dosage,
hydrolysis time and temperature, respectively. The procedure can be used as an environment-friendly alternative to the
traditional procedure of wet alkaline treatment.

Key words: enzyme, sesame, peeling, mixture experimental design