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Qualitative Analysis of Pectinase and β-Glucosidase Secreted by Saccharomyces cerevisiae Strains Isolated from Musalais

XUE Ju-lan 1,2,CHENG Yu-lai1,ZHANG Bai-qing1,ZHU Li-xia2,*   

  1. 1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China;
    2. College of Life Science, Tarim University, Alaer 843300, China
  • Online:2013-10-15 Published:2013-09-27
  • Contact: ZHU Li-xia

Abstract:

Polygalacturonic acid agar medium and Esculin glycerol agar (EGA) medium were used to qualitatively analyze
the pectinase and β-glucosidase secreted by Saccharomyces cerevisiae strains isolated from Musalais. Results showed that
436 wild strains isolated from different manufacturers (or traditional workshops) demonstrated a low or medium ability
to secret pectinase and β-glucosidase. Compared to 5 commercial wine yeast strains, 21 wild strains from modernized
manufacturers were able to produce pectinase with higher activity while 10 strains with high β-glucosidas activity were
mainly from the local workshops. Our results revealed that brewing process and environment influenced the release of
extracellular enzymes from wild yeast.

Key words: Musalais;Saccharomyces cerevisiae;pectinase;&beta, -glucosidase

CLC Number: