Abstract:

Objective: To study the effect of oxidized konjac glucomannan (OKGM) on duodenal morphology and intestinal
flora in C57BL/6J mice fed high-fat diet. Methods: A total of 60 male C57BL/6J mice were randomly divided into 5 groups:
the normal control group (CL, basal diet), high-fat group (HF, high-fat diet) and high-fat diet with low, middle and high
dose OKGM (HF-LOKGM, 12.5g/kg; HF-MOKGM, 25.0g/kg; HF-HOKGM, 50.0g/kg) groups. The mice were killed
after 12 weeks to determine the duodenal villus height and crypt depth, to explore the effect of OKGM on intestinal flora in
C57BL/6J mice fed high-fed diet. Results: The duodenum villus height in the CL group was significantly greater than other
groups (P < 0.05), and there was no significant difference between HF group and HF-LOKGM group (P ＞ 0.05), but the
HF, HF-MOKGM and HF-HOKGM groups revealed a significant difference (P ＜ 0.05). The duodenum crypt depth in the
HF group was significantly larger than other groups (P ＜ 0.05), and a significant difference among the CL, HF-HOKGM
and HF-MOKGM groups was observed (P ＞ 0.05). Similarly, a significant difference in the ratio of villus height and crypt
depth was also observed (P ＜ 0.05). The intestinal flora in the HF-OKGM group was more similar to the CL group, the
diversity index of the HF-HOKGM group was significantly larger than that of the HF group (P ＜ 0.05), but the difference
among the HF-LOKGM, HF-MOKGM and HF groups was not significant (P ＞ 0.05). Conclusion: OKGM can improve
intestinal villus morphology and intestinal flora in C57BL/6J mice fed high-fat diet.

Key words: oxidized konjac glucomannan, C57BL/6J mice, duodenal morphology, denaturing gradient gel electrophoresis, intestinal flora