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Synthesis and Identification of HP-β-CD Inclusion Complex of Grape Seed Extract

WU Chun-ning,LIN Xiang-yang,WANG Chang-chun,ZHENG Wei-dong,YE Nan-hui,WU Jia   

  1. College of Biology Science and Engineering, Fuzhou University, Fuzhou 350108, China
  • Online:2013-10-25 Published:2013-09-28

Abstract:

The preparation of inclusion complex of grape seed extract (GSE) with HP-β-CD was optimized by orthogonal
array design based on one-factor-at-a-time experiments. Incubation in a 20% aqueous solution of HP-β-CD at 40 ℃ for 2 h
was found optimal for the preparation of GSE inclusion complex. The interaction between HP-β-CD and GSE was analyzed
by IR spectroscopy (FT-IR), field emission scanning electron microscopy (FESEM) and X-ray diffraction (XRD). The
aqueous solubility of GSE was increased to 251.5 μg/mL from 87.91 μg/mL as a result of this inclusion treatment, which
was accompanied by a remarkable improvement in its stability.

Key words: grape seed extract (GSE);HP-&beta, -CD;inclusion complex