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Determination of Phenolic Acids in Rapeseed Oil Using Ultra Performance Liquid Chromatography

ZHENG Chang,YANG Mei,HUANG Feng-hong,ZHOU Qi,CAO Xiao-wei   

  1. Hubei Key Laboratory of Lipid Chemistry and Nutrition, Institute of Oilcrops Research,Chinese Academy of Agricultural Sciences, Wuhan 430062, China
  • Online:2013-10-25 Published:2013-09-28

Abstract:

An ultra performance liquid chromatography (UPLC) method for the determination of phenolic acids in rapeseed
oil was established by optimizing solid phase extraction parameters and chromatographic separation conditions. Five
phenolic acids such as sinapine, caffeic acid, sinapic acid, ferulic acid and cinnamic acid could be effectively separated by
this method. Quantification was performed by an external standard method. A good linear relationship was observed for five
phenol acid standards with correlation coefficients of 0.9994 to 0.9999. The detection limits were in the range of 0.07
to 0.11 mg/L. The average recovery rates of five phenolic acids in rapeseed oil were 100.6% to 106.7% with relative standard
deviations less than 2.9%. The method had the advantages of less interference from impurities and high recovery and
sensitivity. Phenolic acid composition of cold-pressed rapeseed oil (CPRO) with high, medium and low contents of erucic
acid was determined by using this method, and analysis of variance showed significant differences in the contents of phenolic
acids among high, medium and low-erucic acid CPROs.

Key words: phenolic acid, rapeseed oil, ultra performance liquid chromatography (UPLC), solid-phase extraction(SPE)