Isolation and Monosaccharide Composition Analysis of Indigenous Sugarcane Polysaccharide

doi:10.7506/spkx1002-6630-201320038

Abstract

Abstract:

Indigenous sugarcane polysaccharide (ISP) was obtained from four clarified juice from different cultivars of sugarcane
by ethanol precipitation. The ISP content was determined using phenol-sulfuric acid method and the ISP composition
after acid hydrolysis was analyzed by gas chromatography. The results showed that the ISP content in the clarified sugarcane
juice from industrial production (a hybrid from three cultivars) was higher than in the laboratory prepared ones (from three
other cultivars, respectively). The ISP monosaccharide composition of all the clarified sugarcane juices was the same, including
ribose, rhamnose, arabinose, xylose, mannose, glucose and galactose. The above results suggested that the ISP in the
clarified sugarcane juice mainly derived from the hemicelluloses and celluloses in the sugarcane fiber. The relative contents
of the monosaccharides had a significant difference among these four sugarcane juices.

Key words: sugarcane, indigenous sugarcane polysaccharide (ISP), isolation, monosaccharide composition

Ban Jia，Chen Jun-jia，Hu Xue-mei，XU Xiao-yan，FU Jin-guo，MENG Fei，Wang Fan. Isolation and Monosaccharide Composition Analysis of Indigenous Sugarcane Polysaccharide[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201320038.

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Isolation and Monosaccharide Composition Analysis of Indigenous Sugarcane Polysaccharide

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