FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (22): 5-9.doi: 10.7506/spkx1002-6630-201322002

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Ultrasonic Extraction and Composition Identification of Saponins from Aralia continentalis Leaves

FENG Ying,SUN Shi-hui,ZHAO Lin-na,FAN Wen-li   

  1. 1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China;
    2. College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
  • Received:2013-06-29 Revised:2013-10-12 Online:2013-11-25 Published:2013-12-05

Abstract:

One-factor-at-a-time method and L9(34) orthogonal array design were employed to optimize the extraction process
of saponins from Aralia contenentalis leaves. The results showed that the optimal conditions of ultrasonic extraction
were determined as follows: ethanol concentration of 70%, material-to-liquid ratio of 1:50 (g/mL), extraction duration of 30 min
and ultrasonic power of 200 W. Under the optimal extraction process, the extraction yield of saponins was up to 26.78 mg/g. Macroporous
adsorption resin HPD-100, silica gel, SephadexLH-20 and preparative HPLC were used to separate the extracted
saponin and congmuyenoside A was identified by NMR.

Key words: Aralia contenentalis, saponin, ultrasonic, extraction, identification

CLC Number: