Preparation and Immunoregulatory Activity of Carboxymethyl Polysaccharide from the Seeds of Plantago asiatica L.

doi:10.7506/spkx1002-6630-201322003

Abstract

Abstract:

Polysaccharide derived from the seeds of Plantago asiatica L. (PLP) was carboxymethylated in aqueous alkaline
medium of sodium hydroxide (NaOH) using monochloroacetic acid (MCA) as etherifying agent. The effects of reaction
parameters such as NaOH concentration, the amount of added MCA, temperature and reaction time were investigated using
single-factor and orthogonal array design. Temperature effect on the reaction was not significant. Caboxymethylated PLP
(CM-PLP) with the highest degree of substitution (DS) was obtained in 4.5 mol/L aqueous NaOH with the addition of 2.835 g
of MCA at 50 ℃. Meanwhile, CM-PLPs with high DS showed a significantly enhanced effect on IL-12p70 secretion by
dendritic cells (DCs). This study indicates a new approach to exploit the resource of PLP.

Key words: Plantago asiatica L., polysaccharide, carboxymethylation, dendritic cell, biological activity

CLC Number:

TS201.2

JIANG Le-ming，FAN Can-mei，NIE Shao-ping，ZHOU Hua-lu，LI Jian-yu，XIE Ming-yong. Preparation and Immunoregulatory Activity of Carboxymethyl Polysaccharide from the Seeds of Plantago asiatica L.[J]. FOOD SCIENCE, 2013, 34(22): 10-14.

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