FOOD SCIENCE ›› 2013, Vol. 34 ›› Issue (22): 15-20.doi: 10.7506/spkx1002-6630-201322004

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Macroporous Resin Adsorption for Purification of Hop Polyphenols from and Compositional Analysis

WANG Xu-ping,YANG Lei,YANG Xiao-lan,LUO Zheng-ming   

  1. 1. College of Life Science, Shanxi University, Taiyuan 030006, China;
    2. College of Life Sciences, Nankai University, Tianjin 300071, China;
    3. Department of Hospital Travel and Tourism Management, Xinzhou Teachers University, Xinzhou 034000, China
  • Received:2013-05-26 Revised:2013-10-25 Online:2013-11-25 Published:2013-12-05

Abstract:

Hop polyphenols were extracted from spent hops, purified by macroporous resin adsorption and subjected to
compositional analysis by HPLC. The results showed that the adsorption and desorption capacities of hop polyphenols on
SP850 resin were 79.8 mg/g and 78.4%, respectively, and the adsorption and desorption processes were equilibrated in a
short time, indicating the suitability of this resin for purifying the crude phenols extracted from hops. The optimal purification
conditions were loading of 6 BV of 7.3 mg/mL sample solution at a flow rate of 3 BV/h followed by washing with 5 BV
of deionized water and elution with 4 BV of 60% ethanol at a flow rate of 2 BV/h. Under the optimized conditions, the purity
of polyphenols in hop extracts was increased from 13.2% to 56.6%. The compositional analysis indicated that 55.2% of the
purified polyphenols were proanthocyanidins and 10 individual phenols were identified by HPLC, which were gallic acid,
catechin, chlorogenic acid, epicatechin, rutin, hyperoside, astragalin, resveratrol, isoquercitrin and xanthohumol

Key words: hop polyphenols, macroporous resin, purification, high performance liquid chromatography (HPLC)

CLC Number: