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Optimizing Ultrasonic-Assisted Extraction of Pigment from Lonicera maackii Fruit by

WANG Li-zhi,TANG Ke-hua   

  1. Key Laboratory of Hunan Forest Products and Chemical Industry Engineering, Jishou University, Zhangjiajie 427000, China
  • Online:2013-11-25 Published:2013-12-05

Abstract:

Following a period of spontaneous solvent extraction at a constant temperature, ultrasonication was applied to
enhance pigment extraction from crushed Lonicera maackii fruit. Three extraction parameters including material/solvent ratio,
ultrasonication time and power were optimized by using response surface methodology. All the operating parameters had
a significant effect on extraction efficiency and the optimal extraction conditions were determined as 1:15 (g/mL), 30 min
and 500 W for material/solvent ratio, ultrasonication time and power, respectively. The predicted absorbance at 450 nm of
the pigment extracted under the optimized conditions after 10-fold dilution was 0.836, showing a relative error of only 1.08%
in comparison to the experimental absorbance of 0.827. Thus the optimized process established using response surface methodology
is feasible for pigment extraction from Lonicera maackii fruit.

Key words: Lonicera maackii, pigment, ultrasonic wave extraction, response surface methodology