Extraction of Soluble Dietary Fiber from Apple Pomace and Its Scavenging Capacity against Free Radicals

doi:10.7506/spkx1002-6630-201322015

Abstract

Abstract:

The extraction of soluble dietary fiber (SDF) from Hanfu apple pomace by hydrolysis with cellulose after pretreatment
with α-amylase and papain was optimized by response surface methodology using a Box-Behnken design on the basis of onefactor-
at-a-time experiments. Four variables influencing the extraction process, such as enzyme dosage, initial pH, temperature
and time were investigated. At the same time, the scavenging effects of apple pomace SDF against DPPH, superoxide anion and
hydroxyl free radicals were evaluated. The results indicated that the optimal extraction conditions were 1.06%, 4.9, 48 ℃ and
228 min for enzyme dosage, initial pH, temperature and time, respectively. The yield of SDF under the optimized conditions was
(18.21±0.21)%. The SDF obtained was able to scavenge all the three free radicals.

Key words: Hanfu apple pomace, soluble dietary fiber, extraction, free radical

ZHAO Ming-hui，Lü Chun-mao，MENG Xian-jun，HAN Lu，ZHENG Peng. Extraction of Soluble Dietary Fiber from Apple Pomace and Its Scavenging Capacity against Free Radicals[J]. FOOD SCIENCE, 2013, 34(22): 75-80.

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