Determination of Polycyclic Aromatic Hydrocarbons (PAHs) in Vegetable Oil by Gas Chromatography Coupled with Triple Quadrupole Mass Spectrometry Using Isotope Dilution

doi:10.7506/spkx1002-6630-201322041

Abstract

Abstract:

An analytical method for the determination of 16 types of polycyclic aromatic hydrocarbons (PAHs) in vegetable
oil has been developed. Samples that had been spiked with isotope standard solution were extracted using acetonitrile/acetone
(60:40, V/V) and purified by gel permeation chromatography (GPC). Identification and quantification were performed
using gas chromatography-triple quadrupole fandem mass spectrometry (GC-MS-MS) in the selected reaction monitoring
(SRM) mode. The efficiency of GPC was compared with that of solid phase extraction (SPE) for purifying crude extracts
and the instrument parameters were optimized. The recovery rates of 16 PAH standards were in the range of 70.8%–123.1%
from samples spiked with 4–6 μg/kg internal standard. The precision (RSD) of the developed method was 1.0%–6.5% with a
limit of quantification of 0.22–0.74 μg/kg (r ≥ 0.9982). Therefore, it is suitable for the analysis of 16 types of PAHs.

Key words: vegetable oil, gel permeation chromatography (GPC), isotope dilution, gas chromatography-triple quadrupole tandem mass spectrometry (GC-MS-MS), polycyclic aromatic hydrocarbons (PAHs)

CLC Number:

ts201.6

YANG Jun，WANG Jian-hua，GONG Ping，LIU Jing-jing. Determination of Polycyclic Aromatic Hydrocarbons (PAHs) in Vegetable Oil by Gas Chromatography Coupled with Triple Quadrupole Mass Spectrometry Using Isotope Dilution[J]. FOOD SCIENCE, 2013, 34(22): 202-207.

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