Reactive Oxygen Species (ROS) Regulates Listeria monocytogenes Biofilm Formation

doi:10.7506/spkx1002-6630-201323040

Abstract

Abstract:

In order to explore the role of ROS (reactive oxygen species) in Listeria monocytogenes (LM) biofilm formation,
we added either diphenyleneiodonium chloride (DPI) as a NADPH oxidase inhibitor or N-acetylcysteine (NAC) as an
antioxidant in combination with exogenous H2O2 to observe the changes of ROS and biofilm formation. The results showed
that both DPI and NAC were able to restrain the biofilm formation of LM by reducing the amount of ROS generation. To
some extent, the addition of H2O2 could promote biofilm formation. Our results suggest that NADPH oxidase exists in LM,
which is responsible for generating ROS. ROS plays a vital role in LM biofilm formation and may be an important signal
molecule.

Key words: reactive oxygen species (ROS), Listeria monocytogenes, biofilm

CLC Number:

Q932

ZHANG Chao，CHEN Guo-wei，YANG Yu-ping，WU Shu-yan，LIU Qing*. Reactive Oxygen Species (ROS) Regulates Listeria monocytogenes Biofilm Formation[J]. FOOD SCIENCE, 2013, 34(23): 189-193.

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