Cellulase Production from Tea Seed (Camellia oleifera) Cake Using Neurospora crassa Mixed with Other Bacteria

doi:10.7506/spkx1002-6630-201401034

Abstract

Abstract:

The production of cellulaes from tea seed (Camellia oleifera) cake with a minimized content of residual tea
saponin of 0.917% as obtained by ultrasonic extraction method was studied in solid-state fermentation by mixed strains of
Neurospora crassa with Issatchenkia oriental, Trichoderma reesei, Trichoderma vivide or L. plantarum. The activities of
cellobiohydrolase 1 (C1), β-1,4-endoglucanase (Cx) and β-glucosidase (β-G) and filter paper activity (FPA) in fermentation
supernatants were determined to explore their synergistic action on enzymatic hydrolysis of cellulose. The enzyme activity of
C1 from Neurospora crassa combined with Trichoderma vivide was increased by 51.09% compared with that obtained from
single-strain fermentation by Neurospora crassa; meanwhile, the combination of both strains resulted in prolonged secretion
of cellulases, and the FPA activity reached 2.782 U/g after 96 h. The cellulose was degraded by 64.19% and 61.59% in tea
seed cake fermented for 10 days by Neurospora crassa mixed with Trichoderma reesei and Trichoderma vivide, respectively.
The overall tendency of the enzyme activities was an increase with increasing the inoculum size irrespective of fermentation
with Neurospora crassa alone or combined with other strains. However, the cellulase activities were declined at higher
inoculum sizes surpassing 9 mL/100 g of Neurospora crassa when inoculated alone. These results indicate that Neurospora
crassa is able to cooperate with Trichoderma reesei and Trichoderma vivide respectively to degrade cellulose.

Key words: Neurospora crassa, cellulases, mixed-bacteria fermentation, solid-state fermentation, tea seed

CLC Number:

TS209

LIU Pei-yi, DENG Ze-yuan, YANG Jian-yuan, LI Jing*. Cellulase Production from Tea Seed (Camellia oleifera) Cake Using Neurospora crassa Mixed with Other Bacteria[J]. FOOD SCIENCE, 2014, 35(1): 174-179.

References

[1] 邓桂兰，魏强华. 利用茶粕生产菌体蛋白饲料的研究[J]. 粮食与食品工业. 2008,15:31-38

[2] 黄卫文, 敖常伟, 钟海雁. 油茶皂素抑菌效果研究[J]. 经济林研究, 2002, 20( 1): 17-19.

[3] 黄冬梅，香私，李庆，等. 茶皂素提取工艺的研究及应用前景. 化工科技市场，2003，(11)：11?13.

[4] 陈钦, 郑清芳. 油茶饼综合利用的研究[J]. 福建林学院学报, 2002, 20(2): 97～100.

[5] 罗永生，常春. 固态混菌发酵产纤维素酶的研究进展[J]. 酿酒科技2010 No.1(187)

[6] Charilaos Xiros, Evangelos Topakas, Petros Katapodis, Paul Christakopoulos. Hydrolysis and fermentation of brewer’s spent grain

　　by Neurospora crassa[J]. Bioresource Technology 99 (2008) 5427–5435

[7] 熊海燕,王卫国,王存文,徐汶. 混合菌培养及其在工业上的应用[J]. 贵州化工. 2004,29(3)：16-19..

HaiKuan Wang et,al *, YuJie Dai. Genome shuffling of Lactobacillus plantarum for improving antifungal activity[J].Food Control 32 (2013) 341-347

[8] S.Rodriguez Couto, M A.Sanroman. Application of solid-state fermentation to ligninolytic enzyme production[J].Biochemical Engineering Journal.2005,22：211-219.

[9] 考书娟, 张锋, 张彬. 丝状真菌YW-7与乳酸菌共同培养发酵豆粕的研究[J]. 家禽科学 2008.6

[10] 张昆，王春维，余岳，彭凯. 复合杂粕多菌种发酵工艺研究[J]. 粮食与饲料工业 2012. 5(49-52)

[11] Aftab Ahamed, Patric Vermette. Enhanced enzyme production from mixed cultures of Trichoderma reesei RUT-C30 and Aspergillus niger LMA grown as fed batch in a stirred tank bioreactor[J]. Biochemical Engineering Journal,2008,42：41-46.

[12] 肖玉娟，邓泽元* ，范亚苇，李静，刘蓉，胡蒋宁. Neurospora.crassa降解茶粕培养基粗纤维的发酵工艺研究[J]. 食品科学. 2010, 31(23):243-247

[13] Harinder Singh Oberoi, Yogita Chavan, Sunil Bansal ,Gurpreet Singh Dhillon. Production of Cellulases through Solid State Fermentation Using Kinnow Pulp as a Major Substrate. Food Bioprocess Technol (2010) 3:528–536

[14] Seonghun Kim ? Chul Ho Kim. Production of cellulase enzymes during the solid-state fermentation of empty palm fruit bunch fiber[J]. Bioprocess Biosyst Eng (2012) 35:61–67

[15] 陈贤情商晋宋慧芳郭康权. 秸秆中纤维素/半纤维素和木质素的几种测定方法对比. 西北农林科技大学

[16] Bungay H R, Bungay M L. Microbial interactions in continuous culture [J]. Advan Appl. Microbiol, 1986, 10: 269-312.

[17] 柳杰，张晖，王立，郭晓娜，等.花生粕固态发酵生产高蛋白饲料菌种的筛选[J].中国油脂，2010,35:16-18.

[18] 曹健，郭德宪，曾实，汪晨辉. 里氏木霉纤维素酶的纯化和性质[J]. 食品科学. 2003, 24(5): 73~75

[19] DE VRIES R P, VISSER J. Aspergillus enzymes involved in degradation of plant cell wall polysaccharides[J].MicrobiolMol Biol Rev,2001,65(4):497-522.

[20] 陈合，余建军，舒国伟，等. 复合酶降解高温蒸煮玉米秸秆的饲料化研究[J]. 食品工业科技，2010（12）：176-178，181.

[21] 戴四发贺淹才绿色木霉纤维素酶系分泌特性及酶解条件的研究[J]. 安徽技术师范学院学报,2001,15(4):50~53

[22] Virendra S. Bisariaa, Tarun K. Ghose (1981). Biodegradation of cellulosic materials: Substrates, microorganisms, enzymes and products. Biochemical Engineering Research Centre, Indian Institute of Technology, Enzyme and Microbial Technology Volume 3, Issue 2, Pages 90–104

[23] Ernestina M. Peraltaa, Hideo Hatate,*, Daisuke Kawabe, Rui Kuwahara, Shinji Wakamatsu,Tamami Yuki, Hisashi Murata. Improving antioxidant activity and nutritional components of Philippine salt-fermented shrimp paste through prolonged fermentation[J]. Food Chemistry. 2008, 111:72–77

[24] P. Prema & D. Smila & A. Palavesam & G. Immanuel. Production and Characterization of an Antifungal Compound (3-Phenyllactic Acid) Produced by Lactobacillus plantarum Strain[J]. Food Bioprocess Technol (2010) 3:379–386