Isolation and Purification of Phenolic Compounds from Tea Seed Oil and Their Antiproliferative Activity on HepG2 Cells

doi:10.7506/spkx1002-6630-201724016

Abstract

Abstract: Phenolic compounds from tea seed oil were extracted with 60% methanol and purified by sequential chromatography on silica gel and Sephadex LH-20 columns. The composition of purified phenolics was determined by RP-HPLC-DAD. Phenolic compounds with antiproliferative effect on HepG2 cells were identified by principal component analysis. The results showed that as one of the three fractions separated by Sephadex LH-20, Fr1 contained 5 phenolic compounds?including catechins (accounting for 62.60% of the total amount) and flavonoids. Fr2 mainly included phenolic acids and flavonoids; flavonoids represented 78.70% of Fr3. IC50 values of Fr1, Fr2, and Fr3 for the proliferation inhibition of HepG2 cells were 1.76%, 16.24%, and 12.35%, respectively. Principal component analysis showed that rutin and catechin were the major constituents in tea seed oil that had a significantly strong ability to inhibit cell viability.

Key words: tea seed oil, phenolic componds, isolation and purification, HepG2, antitumor activity

CLC Number:

TS225.1

WANG Lijuan, LIANG Xingqiu, WANG Xiaoqin. Isolation and Purification of Phenolic Compounds from Tea Seed Oil and Their Antiproliferative Activity on HepG2 Cells[J]. FOOD SCIENCE, 2017, 38(24): 101-106.

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Isolation and Purification of Phenolic Compounds from Tea Seed Oil and Their Antiproliferative Activity on HepG2 Cells

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