Separation and Identification of Ethyl Acetate Extract of Propolis by Reverse Phase-High Performance Liquid Chromatography-Mass Spectrometry

doi:10.7506/spkx1002-6630-201402035

Abstract

Abstract:

The purpose of this study is to separate and identify ethyl acetate extract of propoils from Nanyang, central
China’s Henan province by reverse phase-high performance liquid chromatography-diode array detector compled with
electrospracy ionization-tandem mass spectrometry (RP-HPLC-DAD-ESI-MS-MS). A method for detecting 10 flavonoid
reference standards was established using RP-HPLC-DAD. The linear correlation coefficients for the ten flavonoids were
0.998 2–0.999 9 and the recoveries were 93.40%–103.69%. The RSDs of precision, stability, and spiked recovery were all
less than 5%. The ethyl acetate extract of Henan propolis was analysed by RP-HPLC-DAD-ESI-MS-MS. Totally 11 flavonoids
were identified including quercetin, apigenin, kaempferol, isorhamnetin, chrysin, pinocembrin, galangin, pinobanksin, pinobanksin-
3-O-acetate, pinobanksin-3-O-propionate, pinobanksin-3-O-butyrate and two phenolic acids and their esters.

Key words: propolis, reverse phase-high performance liquid chromatography-mass spectrometry (RP-HPLC-MS), flavonoids, phenolic acids and esters

CLC Number:

TS201.2

WANG Bing1,2, CHEN Yang1, LI Bao-li1, ZHU Yu-xuan1,2, TANG Cui-e1,2, HUANG Yan-chun1, ZHANG Di1, LIU Rui1,2,*. Separation and Identification of Ethyl Acetate Extract of Propolis by Reverse Phase-High Performance Liquid Chromatography-Mass Spectrometry[J]. FOOD SCIENCE, 2014, 35(2): 186-190.

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