FOOD SCIENCE ›› 2014, Vol. 35 ›› Issue (2): 271-276.doi: 10.7506/spkx1002-6630-201402053

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Effects of Different Storage Temperatures on the Activities of Fourteen Enzymes Involved in Sucrose Metabolism in Hami Melon

CAI Xiu-zhen, PANG Guang-chang*   

  1. Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science,
    Tianjin University of Commerce, Tianjin 300134, China
  • Received:2013-06-27 Revised:2013-12-09 Online:2014-01-25 Published:2014-02-19
  • Contact: PANG Guang-chang E-mail:952597220@qq.com

Abstract:

This study aimed to shed light on the respiratory metabolism and sucrose accumulation in Hami melon under
different storage conditions. The activities (gene expression levels) of fourteen enzymes involved in sucrose metabolism
were measured at different storage temperatures (0, 5, 10, 15, 20, 25, 30, 35, 40 and 45 ℃). These enzymes in the epicarp,
endocarp, outer pulp, middle pulp, inner pulp were investigated as well. The results showed that among these enzymes
examined, the activities of invertase and lactate dehydrogenase were the highest. The decreasing order of the two enzyme
activities in five tissues was as follows: endocarp > outer pulp > epicarp > middle pulp > inner pulp. When Hami melon
was stored at 5 ℃, the activities of all 14 enzymes were at a low level. Thus, 5 ℃ is the optimal storage temperature and
sugar metabolism can be controlled effectively at this temperature.

Key words: storage temperature, Hami melon, sucrose metabolism, enzyme activity, respiration

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