Abstract:

Aim: To explore the effect of grape seed proanthocyanidin extract (GSPE) on cis-diamminedichloroplatinum (CDDP)-
induced damage in H9c2 cells and its mechanism. Methods: H9c2 cells were cultured in vitro. The effects of CDDP individually
and in combination with GSPE on the growth of H9c2 cells were determined by MTT method. Meanwhile, the morphologic
change of the cells was observed. SOD activity and MDA content in both cells and medium were determined to explore its
mechanism. Results: GSPE could improve CDDP-induced morphologic change and suppress the decrease in SOD activity as well
as the increase in MDA content in H9c2 cells and culture solutions. Conclusion: GSPE can ameliorate CDDP-induced damage in
H9c2 cells in vitro. Its mechanism may be correlated with increased antioxidant capacity in H9c2 cells.

Key words: GSPE, cis-diamminedichloroplatinum, H9c2 cells, antioxidant