FOOD SCIENCE ›› 2014, Vol. 35 ›› Issue (4): 131-136.doi: 10.7506/spkx1002-6630-201404027

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Determination of 8 Components in Fresh Samples of 3 Scutellaria Species Using On-Line Liquid Chromatography-Tandem Mass Spectrometry

DONG Zhe1, HUANG Xiang-lu1, CAO Jin1,*, ZHANG Qing-sheng1, XIAO Sheng-yuan2, Ian B. Cole3, Susan J. Murch3   

  1. 1. Institute of Food and Cosmetics Control, National Institutes of Food and Drug Control, Beijing 100050, China;
    2. School of Life Science, Beijing Institutes of Technology, Beijing 100081, China;
    3. Chemistry, I.K. Barber School of Arts and Sciences, University of British Columbia Okanagan, Kelowna V1V1V7, Canada
  • Received:2013-04-25 Revised:2014-01-22 Online:2014-02-25 Published:2014-03-17
  • Contact: Jin Cao E-mail:cao1208@gmail.com

Abstract:

Objective: An on-line liquid chromatography-tandem mass spectrometry method was developed to determine 8
components in fresh Scutellaria samples from three germplasm sources. Methods: Mixtures of homogenated tissue samples and
appropriate amounts of packing material were packed in sample chamber. After removing matrix impurities and enriching the
target analytes, 8 components in fresh Scutellaria samples were determined. Results: The recoveries were greater than 90% at the
limits of detection and quantification. Meanwhile, the RSDs of repeatability and stability were less than 3.1%. Conclusion: The
method developed can be used for the determination of active components in Scutellaria samples.

Key words: liquid chromatography-mass spectrometry (LC-MS), Scutellaria baicalensis Georgi, Scutellaria lateriflora L., Scutellaria racemosa Pers.

CLC Number: