Determination of Arsenic, Mercury and Lead in Radix Puerariae by Atomic Florescence Spectrophotometry with Wet Digestion

doi:10.7506/spkx1002-6630-201404031

Abstract

Abstract:

A method for determination of the contents of heavy metals including As, Hg and Pb in 3 batches of Radix
Puerariae (Pueraria thomsonii Benth cultivar “Xiangge No.1” grown in Hunan province harvested at different times) was
developed using atomic fluorescence spectrophotometry in combination with wet digestion. Samples were wet digested by
nitric acid and perchloric acid and then detected by hydride generation atomic florescence spectrophotometry. The linear
ranges for As, Hg and Pb were 0－16.0, 0－5.0 and 0－40.0 ng/mL, respectively, with correlation coefficients of 0.999 9,
0.999 0 and 0.999 7, respectively. Their limits of detection were 0.050, 0.012 and 0.038 μg/L (n = 11), respectively. Their
recovery rates were 98.73%, 101.22% and 99.43% (n = 6), respectively. This method allows simple, fast and accurate
determination of As, Hg and Pb in Radix Puerariae.

Key words: wet digestion, atomic fluorescence spectrophotometry, Pueraria thomsonii Benth cultivar “Xiangge No.1”, As, Hg, Pb

CLC Number:

R284.1

YE Hui-xuan1, TAN Zhou2, LIU Xiang-qian1,*, ZHOU Bin-wen2, XIONG Li-fu3. Determination of Arsenic, Mercury and Lead in Radix Puerariae by Atomic Florescence Spectrophotometry with Wet Digestion[J]. FOOD SCIENCE, 2014, 35(4): 151-154.

References

[1]刘喜舞, 戴玲, 叶惠煊, 等.法同时测定中国、泰国葛根中异黄酮的含量[J].中国药师, 2013, 16(5):668-670

[2]尤春雪, 张振秋, 李峰, 等.波长切换技术对葛根中种成分的测定及指纹图谱研究[J].中草药, 2013, 44(5):616-621

[3] 熊力夫, 熊劲雅, 刘益群.葛根规范化栽培技术研究[J].湖南农业科学, 2010, 11:31-33

[4]熊力夫, 邹亲朋, 叶惠煊, 等.湘葛一号葛根活性物质提取制备葛根功能饮品研究[J].湖南林业科技, 2013, 40(4):33-38

[5]孙艳, 赵余庆.药食同源品中重金属的检测方法与思考[J].中草药, 2011, 42(11):2351-2359

[6] 崔兰冲, 沈晓君, 蔡广知, 等.色谱-原子荧光光谱联用技术测定北柴胡中砷的含量[J].中国实验方剂学杂志, 2013, 10:112-114

[7]高向阳, 韩帅, 王莹莹, 等.微波消解-氢化物原子荧光光谱法同时测定贼小豆中的砷、硒、锑[J].食品科学, 2013, 34(10):215-218

[8] Wu Yanfang, Wang Xinsheng, Fan Enguo.Optimisation of Ultrasound-assisted Extraction of Puerarin and Total Isoflavones from Puerariae Lobatae Radix (Pueraria lobata (Wild.) Ohwi) with Response Surface Methodology[J].Phytochemical Analysis, 2012, 23:513-519

[9] Zhao Chenxi, Chan Hoi-yan, Yuan Dalin, et al.Rapid Simultaneous Determination of Major Isoflavones of Pueraria lobata and Discriminative Analysis of its Geographical Origins by Principal Component Analysis[J].Phytochemical Analysis, 2011, 22:503-508

[10] Yusakul Gorawit, Putalun Waraporn, Udomsin Orapin, et al.Comparative analysis of the chemical constituents of two varieties of Pueraria candollei[J].Fitoterapia, 2011, 82:203-207

[11] Satoko Shimokawa, Takuya Kumamoto, Tsutomu Ishikawa, et al.Quantitative analysis of miroestrol and kwakhurin for standardization of Thai miracle herb ‘Kwao Keur’ (Pueraria mirifica) and establishment of simple isolation procedure for highly estrogenic miroestrol and deoxymiroestrol[J].Nat Prod Res, 2013, 4-5(27):371-378

[12] Rowan F.Sage, Heather A.Coiner, Danielle A.Way, et al.Kudzu [Pueraria montana (Lour.) Merr.Variety lobata]: A new source of carbohydrate for bioethanol production[J].BIOMASS BIOENERG, 2009, 33:57-61

[13]C.Boonchird,T,Mahapanichkul,WCherdshewasart. Differential binding with ERá and ERa of the phytoestrogen-rich plant Pueraria mirifica[J].Braz J Med Biol Res, 2010, 2(43):195-200

[14] Latiporn Udomsuk, Thaweesak Juengwatanatrakul, Waraporn Putalun, et al.Down regulation of gene related sex hormone synthesis pathway in mouse testes by miroestrol and deoxymiroestrol[J].Fitoterapia, 2011, 82:1185-1189

[15]肖冰心, 王倩, 樊利青, 等.葛根黄酮提高桑白皮降糖活性及其机制研究[J].中国实验方剂学杂志, 2013, 19(3):179-183

[16]Niu Ling, Li Dongye, Xia Yong, et al.Effect of Puerarin on the myocardial perfusion and ventricular wall motion in patients with acute coronary syndrome[J].J Geriatr Cardiol, 2008, 5(2):50-53

[17] Chang Yi, Heieh Chengying, Peng Ziaa, et al.Neuroprotective mechanisms of puerarin in middle cerebral artery occlusion-induced brain infarction in rats[J].J Biomed Sci, 2009, 16:9-21

[18] 中华人民共和国对外贸易经济合作部.药用植物及制剂进出口绿色行业标准(WM2-2001) [S].

[19]何毅, 叶正良, 赵利斌, 等.中美欧中药植物药传统草药质量标准差异分析[J].中草药, 2012, 43(1):182-186

[20]刘家明, 周志强, 祖元刚, 等.湿法消解-火焰原子吸收法测定动物样品中六种金属元素[J].光谱学与光谱分析, 2012, 32(7):1961-1963

[21]韩静, 肖伟, 陈芳, 等.不同蔬菜对重金属汞、铬、砷、镉、铅积累效应的研究[J].中国农学通报, 2012, 28(21):264-268