Purification of Total Flavonoids Extracted from Ponkan (Citrus reticulata Blanco cv. Ponkan) Peel by AB-8 Macroporous Adsorption Resin and Separation of Flavonoid Constituents

doi:10.7506/spkx1002-6630-201406006

Abstract

Abstract:

Objective: To propose an optimal purification process for flavonoids extracted from ponkan (Citrus Reticulata
Blanco cv. Ponkan) peel. Methods: The dynamic adsorption and desorption performance of AB-8 macroporous resin towards
flavonoids extracted from ponkan peel was investigated. Major process parameters including sample concentration, pH,
flow rate, and elution ethanol concentration were evaluated. Then the purified flavonoids were isolated by chromatographic
methods with silica gel and semi-preparative HPLC, and the structures were elucidated on the basis of physicochemical
properties and spectral data analysis. Results: The optimal purification process was determined as follows: samples at pH 3.0
containing 3.03 mg/mL ponan peel flavonoids were loaded onto the column at a flow rate of 3.0 BV/h, and then 90% ethanol
was passed through the column to elute the bound flavonoids. After the purification, the purity of ponan peel flavonoids
was increased from 17.8% to 63.1%. In addition, eight compounds were isolated from the purified flavonoids and identified
as tangeretin, nobiletin, 4′,5,7,8-tetramethyl-O-isoscutellarein, 5-demethyl-tangeretin, sinetsetin, aurantine, naringin, and
hesperidin. Conclusion: AB-8 macroporous resin has a high performance in enriching and purifying flavonoids from ponkan
peel, providing a simple and feasible method. Eight compounds can be isolated from the purified flavonoids. Moreover,
4′,5,7,8-tetramethyl-O-isoscutellarein, 5-demethyl-tangeretin, aurantine, naringin, and hesperidin are first reported from
ponkan peel.

Key words: Citrus reticulata Blanco cv. Ponkan peel, flavonoids, AB-8 macroporous adsorption resin

CLC Number:

Q946.8
R284

LUO Dang-wei, YE Jing, HUANG Ya-yan, XIAO Mei-tian*. Purification of Total Flavonoids Extracted from Ponkan (Citrus reticulata Blanco cv. Ponkan) Peel by AB-8 Macroporous Adsorption Resin and Separation of Flavonoid Constituents[J]. FOOD SCIENCE, 2014, 35(6): 30-35.

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