Optimization of Two-Step Enzymatic Hydrolysis of Oviductus Ranae Protein by Orthogonal Array Design

doi:10.7506/spkx1002-6630-201406007

Abstract

Abstract:

Objective: To optimize the two-step enzymatic hydrolysis of Oviductus Ranae protein. Methods: Oviductus
Ranae protein was extracted by soaking in citric acid, homogenizing Oviductus Ranae and then decoting the homogenate
with water, and sequentially hydrolyzed with pepsin and alkaline protease. Various hydrolysis parameters were optimized
by single factor and orthogonal array design methods to obtain maximum percentage of peptides with molecular weight less
than 5 000. Results: The optimum hydrolysis conditions were obtained as follows: pepsin hydrolysis at an enzyme/substrate
ratio of 1:2 000 (m/m) for 2 h followed by alkaline protease hydrolysis at an enzyme/substrate ratio of 1:100 (m/m) for 6 h.
The percentage of peptides with molecular weight less than 5 000 in the hydrolysate obtained under these conditions was
as high as 94.85%, and the protein content of the hydrolysate was 49.85%. Conclusion: The combined use of pepsin and
alkaline protease can effectively decompose Oviductus Ranae protein for better absorption by the body.

Key words: Oviductus Ranae protein, pepsin, alkaline protease, double enzyme method

CLC Number:

R284.2

DONG Ying, ZHANG He, ZHOU Guang-xin, ZHAO Da-qing*, ZHAO Yu, CONG De-yu. Optimization of Two-Step Enzymatic Hydrolysis of Oviductus Ranae Protein by Orthogonal Array Design[J]. FOOD SCIENCE, 2014, 35(6): 36-39.

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