Product Analysis of Plantago asiatica L. Polysaccharide by Acid Hydrolysis

doi:10.7506/spkx1002-6630-201406012

Abstract

Abstract:

Polysaccharides were isolated from seeds of Plantago asiatica L. by water extraction and ethanol precipitation, and
subjected to protein removal by Sevag method, and then the polysaccharide sample was hydrolyzed with 0.3 mol/L TFA by partial
acid hydrolysis for different durations. An ultra performance liquid chromatography coupled with evaporative light scattering
detection (UPLC-ELSD) method was established for establishing better hydrolysis conditions, and ultra performance liquid
chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was used for product analysis
of samples under the optimal hydrolysis conditions. Results showed that the content of polysaccharides in the deproteinized
sample was 68.56%. The optimal hydrolysis conditions were found to be hydrolysis with 0.3 mol/L TFA at 90 ℃ for 1.5 h. The
hydrolysate obtained under these conditions contained more degradation products like monosaccharides and oligosaccharides,
as identified by UPLC-Q-TOF-MS. Simultaneously, some pentose and hexose interconnected pieces in branch chains were also
detected. However, multiple hexose connected pieces were not observed.

Key words: Plantago asiatica L., partial acid hydrolysis, ultra performance liquid chromatography-evaporative lightscattering detector, ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry

CLC Number:

TS207.3

HU Hai-tao, WANG Yuan-xing*, ZHANG Juan, DENG Jing. Product Analysis of Plantago asiatica L. Polysaccharide by Acid Hydrolysis[J]. FOOD SCIENCE, 2014, 35(6): 60-64.

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