Abstract:

To study the immunoregulatory effect of polysaccharide from the seeds of Plantago asiatica L. on RAW264.7
cells, a model of inflammation stimulated with lipopolysaccharide (LPS) was established. The phagocytic activity of the cells
was observed by neutral red phagocytosis experiments. At the same time, the levels of TNF-α, IL-10 and IL-6 in the cultural
supernatant were quantified before and after addition of crude polysaccharide from Plantago asiatica L. (PLCP) by ELISA.
In addition, the expression of NO which was suppressed by the polysaccharide was studied. The results showed that the
polysaccharide could suppress cytokine levels, NO expression and phagocytic activity.

Key words: Plantago asiatica L., polysaccharide, RAW264.7 cells, inflammation, anti-inflammation