FOOD SCIENCE ›› 2014, Vol. 35 ›› Issue (6): 65-68.doi: 10.7506/spkx1002-6630-201406013

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Determination of Erythrosine in Fruit Juice and Candy by Resonance Rayleigh Scattering Method

XUE Jia-xing, LI Chun-yan, LIU Shao-pu, HU Xiao-li*, CUI Zhi-ping, WANG Ya-qiong, TIAN Jing, ZHU Jing-hui, QIAO Man     

  1. Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education,
    College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China
  • Received:2013-07-02 Revised:2014-03-13 Online:2014-03-25 Published:2014-04-04
  • Contact: HU Xiao-li E-mail:xiaolihu@swu.edu.cn

Abstract:

In Britton-Robinson buffer medium at pH 4.2–4.6, nile blue sulphate reacted with erythrosin to form a 1:2 ionassociated
complex, which led to a distinctly enhanced resonance Rayleigh scattering and the appearance of a new resonance
Rayleigh scattering spectrum. The maximum scattering peak was located at 294 nm. The increment of scattering intensity
was directly proportional to the concentration of erythrosin in the range of 0.2 to 4.5 mg/L with the detection limit being
0.007 4 mg/L. Therefore, a sensitive, selective, rapid, accurate and new light scattering method for quantifying erythrosin
was developed. The proposed method was satisfactorily applied to determine erythrosin in fruit juice and candy. The
resonance Rayleigh scattering spectral characteristics and optimal reaction conditions were investigated. The combination
mode of nile blue sulphate with erythrosin was also discussed.

Key words: nile blue sulfate, erythrosme, resonance Rayleigh scattering, juice, candy

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